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Image Search Results
Journal: Advanced Biology
Article Title: Mechanical Loading of Osteocytes via Oscillatory Fluid Flow Regulates Early‐Stage PC‐3 Prostate Cancer Metastasis to Bone
doi: 10.1002/adbi.202400824
Figure Lengend Snippet: Mechanical loading of osteocytes can indirectly regulate PC‐3 cancer cell EMT marker expression and morphology by signaling to endothelial cells. A) Representative immunofluorescent images of vimentin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. B) Quantified mean fluorescence intensity of vimentin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01 (average data). C) Quantified cell aspect ratio of PC‐3 cells in static and OFF groups. Scatter plots show averaged data. (n = 6), overlaid with violin plots of individual cells, n = 600 per group. **p < 0.01 (averaged data) D) Demonstration of a positive correlation between vimentin expression and cell aspect ratio using quantified averaged data. E) Representative immunofluorescent images of E‐cadherin and N‐cadherin staining in PC‐3 cells treated with HUVEC CM. HUVECs were either treated with static or OFF CM from MLO‐Y4 osteocytes. F) Quantified mean fluorescence intensity of E‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.***p < 0.001 (averaged data). G) Quantified mean fluorescence intensity of N‐cadherin staining in PC‐3 cells. Scatter plots show averaged data (n = 6), overlaid with violin plots of individual cells, n = 600 per group. n = 6 samples per group, 100 cells per sample.**p < 0.01.
Article Snippet: Cells were then incubated with a conjugated primary mouse
Techniques: Marker, Expressing, Staining, Fluorescence